Protein production in E. coli
With tailored services and expert guidance, our team will help you define the most relevant strategy for an efficient expression of your protein in the Escherichia coli expression system.
Matchless expertise in E. coli protein expression
GTP Bioways has matchless expertise in Escherichia coli protein expression, gained over 20 years. Our experienced team has successfully produced hundreds of challenging proteins using cytoplasmic, periplasmic and soluble or insoluble (inclusion bodies) expression strategies in Escherichia coli.
Whether your project aims to develop a therapeutic or vaccine protein, obtain materials for research studies or come up with a diagnostic tool, we will be able to provide a customised approach and build efficient processes for the successful production of your target protein in Escherichia coli.
Unique tools for optimised E. coli protein expression
Drawing on our experience expressing a wide range of proteins (enzymes, antigens, cytokines fusion proteins, antibody fragments, etc…), our experts have developed advanced tools for enhanced expression of proteins using E. coli. We have developed our own gene optimisation programme that takes into account parameters that we consider critical for improved protein expression in E. coli and that are usually left aside by gene synthesis companies.
We have also developed our own IP-free vectors with various features designed to improve protein expression and stability.
Unique tools for optimised E. coli protein expression
We have the expertise and capabilities to manage complete E. coli production projects from gene synthesis through to milligram to decagram-scale batch production .
Our custom services include:
- E. coli strain development, including expression vector design & construction
- Upstream process development for soluble/insoluble (inclusion bodies), periplasmic/cytoplasmic expressions
- Downstream process development
- Non-GMP manufacturing for toxicological and stability studies
- Microbial drug substance GMP manufacturing
- Drug product manufacturing through aseptic filling and lot release
GTP Bioways has expertise with other expression systems
We excel at protein production with all prominent industrial microbial, mammalian and insect cell expression systems such as P. pastoris, CHO, HEK and Sf9.
Yeast
Mammalian cells
Insect cells
Are you looking to scale-up or optimise the production of your protein in E. coli?
Connect with our team and we will get back to you shortly.
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